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[35] Vitali G, Parente R, Melotti C.Drugs Exp Clin Res

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

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The detention or removal of nitrating agents may protect against protein inactivation and limit neuronal injury in PD, thus suggesting the necessity of developing therapeutic agents capable of doing this without interfering with normal neuronal function [81]