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LSECs were cultured in supplemented endothelial cell growth medium (ECGM) MV (Promocell, Heidelberg, Germany) with AAS

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In contrast, when tissue folate or cobalamin stores are intact (Silberberg et al, 1997) or in the case of an alteration of the transsulfuration pathway dependent on vitamin B 6 deficiency or on heterozygosity for a CBS defect (Bostom et al, 1995a), an excessive methionine supply might lead to an important elevation of plasma homocysteine